The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. Unplug the power cable from the back of the instrument. Find regional support. Sale. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. StakMax. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Louis, USA). For cell viability assay, cells were incubated for 4 h with 1 mg/ml resazurin solution (10 μl), and then the fluorescence intensity was measured using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 544 nm and emission wavelength of 595 nm . Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Category 2 and 3 microplate readers use gradient heating as well. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. FlexStation™ III. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. book Page 1 Wednesday, July 7, 2010 2:09 PMThe fluorescent intensity was measured using SpectraMax M3 fluorescence microplate reader (Molecular Devices) every 90 seconds with excitation at 320 nm and emission at 420 nm at 37°C for 2 hours. Transfection with siRNA. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . 312 gain calibration failed. Figure 1. 1. com. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The guide also covers the software features, the specifications, and the accessories of the systems. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. 4. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). Supplier: Molecular Devices. The TPC of the samples was calculated by plotting a standard curve with different concentrations of gallic acid (10, 30, 60, 100, and 200 mg/L). 2. e. Figure 2-23: Bottle holder To install the bottle holder: 1. Resolution: The validation protocols are included in the v5. 0200-6117. Type: Clear: Add to Cart. 2. Tumorsphere formation assay and limiting dilution assay. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 306 plate air cal fail,low light. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 115 SpectraMax M4 Multi-Mode Microplate Reader. Microplate Reader Shelf. Add to Cart. Aggregation of MDH was monitored as a function of light scattering at 360 nm over 30 min at 51°C in a SpectraMax M3 (Molecular Devices, United States) microplate spectrometer. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. Molecular Devices SpectraMax M3 User Manual Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. 00 . Light Source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes. l. Detection modalities are shown in Table 1-1. SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The optical density (OD) was read by SpectraMax M3 (Molecular Devices, Sunnyvale, CA). Call 516-939-0888 to check current. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. , Sunnyvale, CA). spr SpectraMax iD5 SpectraTest ABS2 v1. Description 1. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Data obtained in triplicate were calculated and expressed as. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 2. For standard curve values, an albumin standard (Thermo Fisher Scientific) dilution serious. no. ZERO BIAS - scores, article reviews, protocol conditions and moreF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The results were normalized as percentage based. 0 out of 5Growth curves were read in a Spectramax M3 plate reader, at 37 °C with shaking at an optical density of 600 nm; reads were taken every 5 min. Description 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Gemini EM/XPS. No. 314 low lamp level warning. SpectraMax ABS Plus SpectraTest ABS2 v1. It does not work as expected. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Then, adding 2 M sulfuric acid, the reaction was stopped, and the absorbance at 450 nm was determined using a SpectraMax M3 microplate reader (Molecular. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Description 1. 4. Molecular Devices SpectraMax® series microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PM Plate chamber design of the SpectraMax category 1 microplate readers. . SpectraMax® M3. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. AquaMax® 2000. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. Published Dec 16, 2020 | Updated Dec 16, 2020. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. D 1 1. 4. 더 많은. The supernatant was collected after 20 min of incubation in the dark. spr SpectraMax Plus 384. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The mean fluorescence intensity (MFI) was detected with a. 9000-0756. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeNote: In this user guide, SpectraMax ® refers to several SpectraMax . com. NETosis Assays Using Immunostaining and Microscopy. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Filterless readers provide consistent results with steady temperature regulation. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] 21 Chapter 2: Set Up the StakMax Microplate Handler 4. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 6 ARS StainingPatient-derived organoids and xenografts (PDXs) have emerged as powerful models in functional diagnostics with high predictive power for anticancer drug response. An assay plate containing a 15-point standard curve was read on the SpectraMax. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellLuminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. SpectraMax M5/M5e/M4/M3/M2/M2e. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The absorbance was measured at 765 nm using SpectraMax M3 Microplate Reader with SoftMax Pro 7. Fusion of liposomes was followed by content mixing of the RPLs and subsequent increase in fluorescence which was monitored in a SpectraMax M3 fluorescence plate reader (Molecular devices). Page 26 The trademarks used herein are the. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. Please contact Tatiana to reserve the plate reader time: tnl1@psu. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. The chamber. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. Microplate Readers include the M3, M4,. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. Multimode Reader Comparison Chart. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Capture Flash Assays with Ease. With optimized reagents, option to operate the. Moffitt Cancer Center. If no sound is heard during initialization, contact technical support. INTRODUCTION: Proper physiological function relies on the vascular system to distribute oxygenating blood to all tissues, return deoxygenated blood to the lungs, and maintain tissue homeostasis, including functions such as hemostasis, lipid transport, and immune surveillance. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Place the plate drawer transport lock on the end of the plate drawer. Male 8 pin mini Din to Female 9D. The resulting absorbance values were plotted against time. com. StakMax Microplate Handling Stacker. Italic and boldface type are used for emphasis. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). FlexStation™ I. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The intracellular ROS level was also cross-confirmed using the DCF-DA method with a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA,. Luciferase Detection Instrumentation Guide. Total phenolic contents were calculated from the gallic acid standard curve. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0200-7027. Fundamentally, one is interested in whether or not an instrument can detect a signal for a given assay; that is, whether a signal is detectable above the background of the instrument. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. D 1 1. Using the PathCheck® Sensor, results can be automatically normalized to a 1 cm pathlength so that the values are. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Summary of Contents for Molecular Devices SpectraMax M2. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Spectramax PMT voltage? I used spectramax M3 plate reader to measure the fluorescence change through time course. 4 kilograms). Case Study 1 — Molecules Showed High Conformational Similarity: In the first case study, we analyzed multiple batches of innovator Herceptin (trastuzumab) and biosimilar MAbs using the antibody-array ELISA. 2. 7°C (gradient heating design). HNE (EC 3. 4/5 Set up plate template / Define Plate Map 1. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Select wells and then add or select a group or blank and assignThe SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 303 unable to cal dark current. 1. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. We would like to show you a description here but the site won’t allow us. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Examples: “Press carefully to engage,” “ Do not press down. Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. STAKMAX. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. spr SpectraMax iD3 SpectraTest ABS2 v1. Application note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 306 plate air cal. 4. FlexStation™ II. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Impact of fix:Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Cables. Description 1. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. When a feature or capability applies to only certain readers, this exception is noted. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. . 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Our Price: $8,635. S. Fluorescent Polarization: Solid black plates, as above. 0 out of 5 $ 5,999. 2 only) SpectraMax M-series Microplate readers. ; The top 3 importers of Spectramax are India with 365. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5,. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Challenge. Instruments: Cary 630 FTIR, SpectraMax® M3 Multi-Mode Microplate Reader, AR 2000 and TA. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. SpectraMax Mini. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. For more sample throughput, the system canStakMax Handling Stacker Base Unit. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. item 6 White 18-SMD LED License Plate Lights For 04-06 BMW E46 LCI 325Ci 330Ci M3 Coupe White 18-SMD LED License. Carefully place the cytometer on its side on the foam pad so that the top of the cytometer faces the bottom of the microplate reader. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The plates were protected from light and incubated at room temperature for 8 min. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader. For example, microplates containing different samp les can be read using the same or We would like to show you a description here but the site won’t allow us. Next to the pushbutton is a green LED. 1step assay with SpectraMax microplate readers. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). The staphyloxanthin intermediates present in collected supernatant were quantified by measuring at different wavelengths using multi-label reader (Spectramax M3, USA). Subsequently, the PBS solutions containing the cells and the MSNs were incubated at 37°C under shaking at 220 rpm for another 4 h, after which the PBS supernatants were transferred into the wells of a 96-well plate and evaluated on a fluorescent spectrophotometer (SpectraMax M3, Molecular Devices, USA) using an. SpectraMax ® M2 Multi-Mode Microplate Reader. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. Note: In this user guide, all references to SpectraMax Multi-Mode. SpectraMax® ABS Plus. Recertification is recommended every two years. Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. If filters are used with the SpectraMax iD5 reader, integration time may be set to 50 ms, reducing the. The SpectraTest ABS1 Validation Plate package. Plant material. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Also refer to this Multimode Reader Comparison Chart. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email. To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. Cells were cultured for at least 10 days in sphere-forming medium. Photometric accuracy. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 315 can't find zero order. 6: bioNova científica, s. The positive control was treated with fresh RPMI-1640 (Lonza, Walkersville, MD). Turn the instrument around so that the back of the instrument is. Click SoftMax Pro Software User Guide. The concentration of CTC was expressed as mg catechin equivalent per g of extract. spr SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. Offer Ends In: 5 Days 05: 02: 03. Description 1. When a feature or capability applies to only certain readers, this exception is noted. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. Hydrophobicity assayThese settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 4/5 Set up plate template / Define Plate Map 1. Extramitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer SpectraMax® M3 at 540 nm emission and 580 nm. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. 5. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Bioz Stars score: 86/100, based on 1 PubMed citations. Superior flexibilityThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Table 1. ShakerTime: 0 to 999 seconds. Wavelength Range. 4. . The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 3 software. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. The % parasite viability, as indicated by the pLDH activity in the treated wells compared with the untreated controls, was calculated. Qualify the fluorescence performance of the SpectraMax . edu, 814-863-3503. 7 mm for all shake intensities. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. spr * SpectraMax i3(x) SpectraTest ABS2 v1. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. Software Manual – SoftMax Pro. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax® Aquamax. Restart the system. M3-M5e_manual. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. All associated StakMax® stackers. Lower limits of detection for ATP determined using the ATPlite . Molecular Devices Spectramax Plate Reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Tested w/warranty. book Page 1 Tuesday,. The guide also covers the software features, the specifications, and the accessories of the systems. Cells were treated with potassium bromate (30 mM) to provide a pro-oxidant source required to lower the glutathione ratio, which was used as a. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. 1. To assess mast cell degranulation, β-hexosaminidase release was evaluated according to a method published by Dearman et al. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. 7°C and the bottom to 36. 304 signal level saturation. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. A BCIP/NBT ALP staining kit (Beyotime Institute of Biotechnology, Shanghai, China) was utilized to perform ALP staining on the 7th day according to the manufacturer's instructions. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 4. In addition, they allow to increase their performance sequentially from models: M2 ‘ M2e;. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples. 1. Mohd. The actual pathlength, d, of a solvent is found from the following equation: d cm. The innovative and flexible design features. Expanded wavelength coverage. Detection modalities are shown in Table 1-1. For more information, please visit us at using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. Sphere-like cells were subcultured into single-cell suspension, and cells of ≥90% viability were used for sphere formation studies. Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 6 (rated by 49 users) Compatible devices: FilterMax F3, SpectraMax MiniMax 300, SkanWasher 300, Digidata 1550, ClonePix 2, SpectraMax iD3, SpectraMax Paradigm, SpectraMax. The absorbance reading of the purple product was formed, which was an indication of the pLDH activity and, hence, the number of parasites present was quantified in a Spectramax M3 microplate reader (Abs 620). SpectraMax M3, M4, M5, and M5 e. The detection of intracellular ROS and mitochondrial membrane potential SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Cells cultured in 96 well plate are washed with PBS or HBSS (phenol red free), incubated with ~5-10 uM. Fluorescence was measured using a SpectraMax M3 plate reader (excit/emiss = 480/520 nm). Lift the right side of the microplate reader and place the feet into the two mounting cups on the baseplate. 1. The fluorescence of the reaction was read every 3 min (λ excitation = 485 nm, λ emission = 538 nm) for up to 90 min at constant 37 °C with the SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). spr * SpectraMax i3(x) SpectraTest ABS2 v1. The team have compared the effects of antibiotics on bacterial viability and. Page 13 Installing and Setting Up the. The ROS level was detected by a reactive oxygen species assay kit (Beyotime, China). 0 OD for earlier models. Amplitude : 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Example: [Drawer]. Instrument settings used with the SpectraMax M5 Microplate Reader. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. 005) Photometric PrecisionSpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. 316 grating motor driver faulty. XTplusC Texture Analyser Languages: French, English and Greek. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. The assay was repeated at varying final concentrations (5, 15, 25, 50, 100 nM) of PES. We would like to show you a description here but the site won’t allow us. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10 The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 313 reference gain check fail. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:The bound CV was dissolved in 30% acetic acid and the absorption was measured in a microplate reader at OD 590 using SpectraMax M3 (Molecular Devices, United States). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 72 MB.